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Salmonella typhiis a bacteria that causes Salmonellosis which is a serious disease in Indonesia and is still endemic. One of the medicinal plants which has medicinal properties is water hyacinth (Eichhornia crassipes(Mart.) Solms) which has the potential to be antibacterial. This study aims to determine the antibacterial activity of water hyacinth leaves on Salmonella typhiATCC 1408 bacteria. The leaves of water hyacinth were extracted by maceration using 70% ethanol solvent. The results of water hyacinth leaf extraction with70% ethanol solvent produced ethanol extract with a yield of 6.9%. Based on the results of the phytochemical screening test, the ethanol extract of water hyacinth leaves showed the presence of saponin, flavonoid, and polyphenol class compounds. The ethanol extract of water hyacinth leaves was tested for antibacterial activity against Salmonella typhiATCC 1408 using the disc diffusion method. The ethanol extract of water hyacinth leaves was then tested for the inhibition zone and produced antibacterial activity with the largest inhibition zone average in the positive control (Tetracycline) with an inhibition zone of 4.62 mm in the weak inhibiting category, at a concentration of 10% with an inhibition zone of 3.5 mm in the weak inhibiting category, and a concentration of 40% with an inhibition zone of 4 mm in the weak inhibiting category. based on the ANOVA test in the Test Statistics table, the significance value of 0.099 is greater than 0.05 so that there is no difference in ingredients and the concentration has a significant effect on the antibacterial activity of water hyacinth leaf extract.