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Ika Yulya Ningtiyas Pengarang Dr. Iswandi, S.Si, M.Farm., Apt Dosen Pembimbing 1 Dr. Iswandi, S.Si, M.Farm., Apt Penguji 2 Apt. Elly Purwati, S.Si., M.Farm-Klin Penguji 1 Apt. Valiandri Puspadina, S.Farm., M.Farm Ketua Penguji

Thiamin HCl is a very volatile vitamin. Its stability is influenced by pH, temperature and processing. One of the sources of Thiamin HCl obtained by Indonesians is rice, which is the staple food in Indonesia. The washing process that is carried out before the cooking process allows Thiamin HCl to dissolve in water. Thiamin HCl is soluble in water and alcohol. Can be damaged by heat, especially in the presence of alkalis. In dry conditions,Thiamin HCl is stable at 100°C for several hours while humidity will accelerate its destruction.This study aims to determine differences in levels of Thiamin HCl in rice washing water with variations in washing water temperatures of 15°C, 28°C, 37°C and 50°C which were carried out quantitatively using UV-Vis Spectrophotometry. The method of analysis of Thiamin HCl levels in rice washing water includes the absorbance test of Thiamin HCl pro analysis, determining the maximum wavelength, determining operating time, and sample testing. The sample used rice circulating in the Tanjung Mojokerto market with variations in the temperature of the washing water for 2 minutes.Based on the research results, the maximum wavelength of Thiamin HCl is 615nm for 10ppm-40ppm. Operational time for 0-15minutes with an absorbance of 0.36. The linearity data analysis results obtained from the equation y = -0,0219+ (0.0216) C with a relation efficiency of 0.9998. Thiamin HCl content in rice washing water with variations in washing water temperature at 15 °C is 0.0444gr /25gr, 28°C is 0.0261gr /25gr, 37°C is 0.0426gr /25gr and 50°C is 0, 0347gr/25gr.From this study, it was concluded that there was a significant difference in vitamin B1 levels between rice washing water and watertemperature variations of 15°C, 28°C, 37°C, and 50°C which were influenced by the temperature of the washing water.